Gap Inc V.GAP

High vs low dose

[KayDay]

I was able to find what levels are used in the 5 dose assay... At least what they used in 1990.  When they first started the screening process they used 10-4M which is the highest possible dose but now they use 10-5M which is lower but obviously not the lowest dose.  It may be that they are using different levels for Gap's tests...



Cell line panel: 60 Lines total

7 Subpanels initially (lung, colon, renal, ovary, melanoma, brain, leukemia)

Lines used at [ 20 passages from master stock

Culture medium RPMI 1640

5% Serum

Cell inoculation densities: 5000-40,000 cells/well (96-well microtiter plate)

Preincubation: 24 h (no drug)

Sample dilutions Routinely 10-4, 10-5, 10-6, 10-7, and 10-8M or as specified

Duplicates performed at all concentrations

T0 and "no-drug" controls included

Drug incubation: 48 h

End point assay: Sulforhodamine B protein stain

M which is the highest possible dose but now they use 10M which is lower but obviously not the lowest dose.  It may be that they are using different levels for Gap's tests...Cell line panel: 60 Lines total

M which is the highest possible dose but now they use 10M which is lower but obviously not the lowest dose.  It may be that they are using different levels for Gap's tests...Cell line panel: 60 Lines total