BioExx Specialty Proteins Ltd BIXZF

RE: RE: The froth issue explained

[gadfly222]

Does "non-solvent" really mean 'spinning water'??

It seems to me the kernal of the issue (no pun intended) is... "enzyme-assisted aqueous extraction"
https://www.bioexx.com/page/science

Research Paper

Enzyme-assisted aqueous extraction of oil and protein from canola (Brassica napus L.) seeds

1. Sajid Latif1,
2. Levente L. Diosady2,
3. Farooq Anwar1,*

Article first published online: 1 AUG 2008

DOI: 10.1002/ejlt.200700319

Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords:

• Aqueous enzyme-assisted;
• Canola (Brassica napus L.);
• Fatty acids;
• Protein;
• Tocopherols

Abstract

The emphasis of this study was to investigate the effect of enzymes on aqueous extraction of canola (Brassica napus L.) seed oil and protein. Four enzymes, Protex 7L, Multifect Pectinase FE, Multifect CX 13L, and Natuzyme, were tested for their effectiveness in releasing oil and protein during aqueous extraction. The enzyme-extracted oil content of canola seeds (22.2–26.0%) was found to be significantly (p <0.05) higher than that of the control (without enzyme) (16.48%). An appreciable amount of protein (3.5–5.9%) originally present in the seed was extracted into the aqueous and creamy phases during aqueous extraction of oil. The physicochemical properties of oils extracted from canola seed by conventional solvent extraction, and aqueous extraction, with or without enzyme addition were compared. Significant (p <0.05) differences were observed in free fatty acid content, specific extinctions at 232 and 270 nm, peroxide value, color (1-inch cell) and concentration of tocopherols (, , and d). However, no significant variation (p <0.05) was observed in iodine value, refractive index (40 °C), density (24 °C), saponification value, unsaponifiable matter and fatty acid composition. A better oil quality was obtained with aqueous extraction (with and without enzyme) than with solvent extraction. While the enzymes enhanced the oil extraction, the oil yield was still significantly (p <0.05) lower than that obtained by solvent (hexane) extraction.

https://onlinelibrary.wiley.com/doi/10.1002/ejlt.200700319/abstract

Here is one for 'soy beans':

Biotechnol Prog. 2010 Mar-Apr;26(2):488-95.

Protein recovery from enzyme-assisted aqueous extraction of soybean.

Campbell KA, Glatz CE.

Source

Dept. of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA.

Abstract

Enzyme-assisted aqueous oil extraction from soybean is a "green" alternative to hexane extraction that must realize potential revenues from a value-added protein co-product. Three technologies were investigated to recover protein from the skim fraction of an aqueous extraction process. Ultrafiltration achieved overall protein yields between 60% and 64%, with solids protein content of 70%, and was effective in reducing stachyose content, with fluxes between 4 and 10 L/m(2) hr. Protein content was limited because of high retention of lipids and the loss of polypeptides below 13.6 kDa. Isoelectric precipitation was effective in recovering the minimally hydrolyzed proteins of skim, with a protein content of 70%, again limited by lipid content. However, protein recovery was only 30% because of the greater solubility of the hydrolyzed proteins. Recovery by the alternative of protein capture on dextran-grafted agarose quaternary-amine expanded bed adsorption resins decreased with decreasing polypeptide molecular weight. Proteins with molecular mass greater than 30 kDa exhibited slow adsorption rates. Expanded bed adsorption was most effective for recovery of proteins with molecular weight between 30 and 12 kDa. Overall, adsorption protein yields were between 14% and 17%.