Sinai Health preprint on antibody longevity for SARS-CoV-2 QuestCap investment in Sinai Health Foundation will pay off big time, 2 preprints in few weeks, not bad. Seems like they are getting ready for phase.
Abstract
https://www.medrxiv.org/content/10.1101/2020.08.01.20166553v1 While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about the mucosal immune response and its relationship to systemic antibody levels. Since SARS-CoV-2 initially replicates in the upper airway, the antibody response in the oral cavity is likely an important parameter that influences the course of infection. We developed enzyme linked immunosorbent assays to detect IgA and IgG antibodies to the SARS-CoV-2 spike protein (full length trimer) and its receptor binding domain (RBD) in serum (n=496) and saliva (n=90) of acute and convalescent patients with laboratory-diagnosed COVID-19 ranging from 3-115 days post-symptom onset (PSO), compared to negative controls. Anti-CoV-2 antibody responses were readily detected in serum and saliva, with peak IgG levels attained by 16-30 days PSO. Whereas anti-CoV-2 IgA antibodies rapidly decayed, IgG antibodies remained relatively stable up to 115 days PSO in both biofluids. Importantly, IgG responses in saliva and serum were correlated, suggesting that antibodies in the saliva may serve as a surrogate measure of systemic immunity.
Competing Interest Statement
Steven J Drews has acted as a content expert for respiratory viruses for Johnson & Johnson (Janssen). Work in the Gingras lab was partially funded by a contribution from QuestCap through the Sinai Health Foundation. Work in the Gommerman lab unrelated to this project has been funded by EMD-Serono, Roche and Novartis.
Funding Statement
This study was supported by an Ontario Together grant and funding from the Canadian Institutes of Health Research (CIHR; #VR1-172711 and VR4-172732). Funding for the development of the assays in the Gingras lab was provided through generous donations from the Royal Bank of Canada (RBC), Questcap and the Krembil Foundation to the Sinai Health System Foundation. The robotics equipment used is housed in the Network Biology Collaborative Centre at the Lunenfeld-Tanenbaum Research Institute, a facility supported by Canada Foundation for Innovation funding, by the Ontarian Government and by Genome Canada and Ontario Genomics (OGI-139). Indirect support for SARS-CoV-2 work in the Toronto Combined Containment Level 3 laboratory was provided by strategic research funds from the University of Toronto and the Temerity Foundation. MO is funded by: OHTN (Ontario HIV Treatment Network), CIHR and the Juan and Stefania Speck Fund. JG is a Canada Research Chair, Tier 1, in Tissue Specific Immunity and is supported by CIHR FDN15992. ACG is the Canada Research Chair, Tier 1, in Functional Proteomics and is supported by CIHR FDN143301. AJJ is supported by a Vanier Canada graduate studentship.
https://www.medrxiv.org/content/10.1101/2020.08.01.20166553v1.full.pdf+html
Author Declarations
I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.
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The details of the IRB/oversight body that provided approval or exemption for the research described are given below:
REB studies #20-044 Unity Health Network; #02-0118-U/05-0016-C, Mount Sinai Hospital; REB study# 23901 University of Toronto; REB studies #01-0138-U and #01-0347-U, Mount Sinai Hospital; REB studies #31593 University of Toronto, #05-0869, University Health Network
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Paper in collection COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv