Theratechnologies Inc T.TH

RE:RE:RE:RE:New PR aroundVM

[qwerty22]

Four observations from the result that I want to consider together.

1) "When SORT1 expression was repressed through specific siRNA-mediated gene silencing (Figure 4A), MMP-9 was also decreased (Figure 4B). SORT1 silencing lead to decreased 3D structures when compared to scrambled siRNA in ES-2 ovarian cancer cells (Figure 4C) and in MDA-MB-231 TNBC cells"

2) "After 12 h, increasing concentrations of TH1904 strongly inhibited in vitro VM whereas unconjugated Doxorubicin or Doxil, up to 20 µM, slightly affected VM"

3)"The unconjugated TH19P01 peptide itself was found not to alter VM (data not shown)"

4)"Pretreatment of MDA-MB-231 cells (Figure 7A) or ES-2 cells (Figure 7B) cells with TH1902 or TH1904 respectively inhibited cell migration. More importantly, specific silencing of SORT1significantly prevented the anti-migratory effect of both conjugates. It is to note that, in our experimental settings, SORT1 silencing did not alter basal cell migration as specifically assessed through impedance values collected through the xCELLigence"

So 1) says silencing SORT1 stops VM. 2) says PDCs also stop VM. 3) says peptide alone has no effect. 4) say PDC impacts cell migration only in the presence of SORT1 but SORT1 silencing doesn't.
To me that's a super interesting result. There may be technical reasons that explain this based on the way the experiments are performed but if it's explained by the MOA of the various molecules it seems to suggest to me there is something different in the action of the PDC. Cell migration is a necessary part of VM and in this set of expts only the PDC has detectable effects on VM and migration (compared to siRNAvor peptide-alone). To me it says the PDC is doing bonus things. If we thought of this from a business perspective a company company like Sortina (the Swedish guys) are trying to develop a SORT1 inhibitor as potential rival. This set of results suggest potentially the PDCs doing something akin to the silencing and then something a little bit more. Just a sniff of that at the moments.