TSX:MSCL - Post Discussion
Post by
db2067 on Nov 25, 2021 11:43am
Satellos' muscle regeneration therapy...
Check out this paper, exciting work here, this is a much higher level of validation than traditional animal models - things are moving ahead quickly with Satellos' muscle regeneration therapy...
Published NOV 2021
Analysis of human satellite cell dynamics on cultured adult skeletal muscle myofibers Peter Feige1, Eve C. Tsai and Michael A. Rudnicki https://ruor.uottawa.ca/bitstream/10393/41609/1/13395_2020_Article_256.pdf
Here, we report that by utilizing primary human myofibers, we can model human muscle stem cell dynamics in a chemically, physically, and cellularly relevant context. This method is amendable to model human muscle resident stem cells including satellite cells and in the future feasibly other resident cell types in muscle such as fibroadipogenic precursors, mesenchymal cells, fibroblast, pericyte, endothelial cells, and tenocytes [44, 45]. Myofibers can be prepared within 3 h of surgical excision and can be cultured ex vivo for at least 8 days which reflects satellite cell expansion, differentiation, and de novo human myofiber formation. The ability to directly assay genetic pathways in human satellite cells in a relevant context provides an exciting opportunity for pre-clinical testing and to develop causative relationships in human satellite cell signalling
Be the first to comment on this post