Study Shows How Transcriptic’s Robotic Cloud Laboratory Removes Bottlenecks From Biology Experiments and
Generates Reproducible, Publishable Data
Results Published in PLOS ONE Report Largest Enzyme Mutant Data Set Generated to Date
Transcriptic, Inc., a robotic cloud laboratory for life sciences, today announced a peer-reviewed study has been published in PLOS ONE that provides an example of how researchers are successfully using
Transcriptic Workcells to automate and streamline their biology research.
In the study, researchers at University of California, Davis used the Transcriptic Robotic Cloud Laboratory to improve a
critical step in the workflow of computational enzyme design – site-directed mutagenesis, a basic but time-consuming process that
is prone to errors. Results from the study show how Transcriptic Workcells automate mutagenesis at a scale and speed necessary for
generating large-scale datasets.
“To the best of our knowledge, the research community has not previously produced, purified, and measured the kinetic constants
of more than 32 mutants in a uniform manner,” said Justin Siegel, Ph.D., Assistant Professor and Head of the Siegel Lab at the UC
Davis Department of Chemistry. “As a result of the team’s work, and the support we received from Transcriptic, we have now
generated an order of magnitude more data than any previous effort, making this the largest dataset of its kind.”
“By offering the Siegel Lab a virtual infrastructure to run the mutagenesis process, we streamlined their protocol, enabling
automated high-throughput and robust mutagenesis via the cloud,” said Max Hodak, CEO of Transcriptic. “This newly generated dataset
is not only an important step forward in enzyme characterization, but the published study is a seminal milestone for Transcriptic.
To our knowledge, it is the first published paper that demonstrates how a cloud-based laboratory can remove unnecessary bottlenecks
from research experiments and generate reproducible and publishable data.”
Alex Carlin, lead scientist of the study and graduate student in the Siegel Lab commented: “From an academic standpoint, it’s
important that we share our protocols with the scientific community. The mutagenesis protocol we used is available to any
Transcriptic customer and will be invaluable for the development of computational enzyme engineering algorithms that provide
insight into the physical basis of enzyme sequence-structure-function relationships.”
“We are looking forward to working with Transcriptic to help us scale from 100 mutants to 1,000 mutants,” Mr. Carlin added.
Paper Cited:
Carlin, D, et al. Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables the Discovery of Structural Features Correlated
with Kinetic Constants. PLOS ONE. 10.1371/journal.pone.0147596. January 27, 2016.
About Transcriptic Inc.
Transcriptic is the robotic cloud laboratory for life sciences. Transcriptic’s revolutionary, automated cellular and molecular
biology platform enables scalable life science research with flexibility and precision. The convenient and simple web interface
allows biologists to control their science and generate data from virtually anywhere in the world. Transcriptic frees researchers
to focus on the creative aspects of their science by providing faster, more reliable and repeatable processes with vastly lower
capital costs than previously possible. More information on Transcriptic can be found at www.Transcriptic.com.
Media Contact:
Bioscribe Inc.
Maurissa Messier, 760-659-6700
Maurissa@bioscribe.com
View source version on businesswire.com: http://www.businesswire.com/news/home/20160216005377/en/