Agilent Technologies Inc. (NYSE:A) and SISCAPA Assay Technologies (SAT)
today announced they have signed a non-exclusive agreement to enable SAT
to provide an end-to-end solution for multiplexed protein quantitation
using a highly automated workflow for SISCAPA LC-mass spectrometry. This
solution will combine SAT’s reagents
for targeted protein quantitation with Agilent’s Bravo
Automated Liquid Handling Platform for sample preparation, along
with liquid chromatography–mass spectrometers (LC/MS) for quantitative
analysis. The agreement expands on an established strategic relationship
between Agilent and SAT, furthering both companies’ ability to enhance
customer workflows.
SAT will provide an integrated solution for targeted protein
measurement. A protocol for addition-only trypsin digestion followed by
anti-peptide immunocapture enrichment has been developed for the Bravo
platform and is complemented by a purpose-designed, intuitive software
interface. The process supports preparation of 400 multiplexed samples
per working day, with sensitive and selective mass analysis carried out
using Agilent’s triple quadrupole mass spectrometry instruments.
SISCAPA assays alleviate challenges of limited specificity and protein
interferences seen with ligand binding assays and anti-protein
immunocapture mass spectrometry. Subjecting the sample to up-front
tryptic digestion and enriching for proteotypic peptides means SISCAPA
assays measure the amount of a protein target in a highly multiplexed
and high-throughput manner, free of such interferences. This greatly
decreases the bottleneck of screening candidate protein biomarkers in
clinical research.
“Agilent is a leader in workflow solutions, including high-precision
sample preparation systems and rugged, sensitive LC/MS systems, and this
partnership offers customers a truly enabling workflow to execute
targeted protein assays with a throughput and precision that were
previously impossible,” said Yvonne Linney, vice president and general
manager for Agilent’s Life Science Solutions Division, which includes
Automation and LC/MS businesses. “Agilent is pleased to partner with
SISCAPA Assay Technologies, the leader in targeted protein quantitation,
to offer researchers an integrated solution with simplified user
interface, high-precision, sensitive quantitation and enhanced
productivity.”
SAT will offer complete systems comprised of its SISCAPA assay reagents
combined with the Bravo platform and software and a broad offering of
Agilent liquid chromatography and LC/MS systems including, but not
limited to, Agilent’s popular Triple Quadrupole LC/MS systems and
RapidFire high throughput LC/MS system.
“This agreement enables our customers to combine the selectivity of
anti-peptide immunocapture and MRM mass spectrometry to carry out
quantitative protein measurement with high precision and minimal user
intervention,” said Selena Larkin, vice president of Marketing and Sales
at SAT. “As part of this complete solution, SAT will offer all the
components required to implement the workflow as well as provide
technical support for assay execution. The seamless end-to-end solution
is now available from SISCAPA Assay Technologies.”
About SISCAPA Assay Technologies, Inc.
SISCAPA
Assay Technologies Inc. (SAT) delivers tools for development of
high-sensitivity, high-throughput specific assays for proteins,
including clinical biomarkers, using SISCAPA® (Stable Isotope
Standards and Capture by Anti-Peptide Antibodies) technology. SISCAPA is
a registered trademark of SISCAPA Assay Technologies Inc. in the United
States and/or other countries. Additional information about SISCAPA
technology, reagents and licensing is available at www.SISCAPA.com.
About Agilent Technologies
Agilent Technologies Inc. (NYSE:A) is the world’s premier measurement
company and a technology leader in chemical analysis, life sciences,
diagnostics, electronics and communications. The company’s 20,600
employees serve customers in more than 100 countries. Agilent had
revenues of $6.8 billion in fiscal 2013. Information about Agilent is
available at www.agilent.com.
On Sept. 19, 2013, Agilent announced plans to separate into two publicly
traded companies through a tax-free spinoff of its electronic
measurement business. The new company is named Keysight Technologies,
Inc. The separation is expected to be completed in early November 2014.
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Copyright Business Wire 2014